Gene | Primer sequence | Annealing temp. | Number of PCR cycles | Product size |
---|---|---|---|---|
Cox-2 | Forward primer: | 55°C | 30 | 536 bp |
5′-GCTTTCTCCAACCTCTCCTACTACA-3′ | ||||
Reverse primer: | ||||
5′-CATGGGAGTTGGGCAGTCA-3′ | ||||
Beta actin | Forward primer: | 60°C | 30 | 265 bp |
5′-AACCCTAAGGCCAACCGTGAAA-3′ | ||||
Reverse primer: | ||||
5′-TCATGAGGTAGTCTGTCAGGTC-3′ |