Table 1 Sequences for PCR primers of indicated genes of interest
From: AICAR inhibits ceramide biosynthesis in skeletal muscle
Gene | Sequences |
|---|---|
|  | 5’-TGGCATTGTTACCAACTGGG |
β-actin | 5’-GGGTCATCTTTTCACGGTTG |
|  | 5’-TACTCAGAGACCTCCAGCTG |
SPT1 | 5’-CACCAGGGATATGCTGTCATC |
|  | 5’-GGAGATGCTGAAGCGGAAC |
SPT2 | 5’-GTATGAGCTGCTGACAGGCA |
|  | 5’-CTGTTCTACTTGGCCTGTTG |
CerS1/Lass1 | 5’-TCATGCAGGAAGAACACGAG |
|  | 5’-TCTTCTCAAAAAGTTCCGAG |
CerS2/Lass2 | 5’-AGTGATGATGAAAACGAATGG |
|  | 5’-TGGCTGCTATTAGTCTGATG |
CerS3/Lass3 | 5’-TCAGGATAAAGTAACCCCAG |
|  | 5’-TGTCGTTCAGCTTGAGTGAG |
CerS4/Lass4 | 5’-AGCAGGCTTCACAGAATTTC |
|  | 5’-CTCCAACGCTCACGAAATTC |
CerS5/Lass5 | 5’-ATGCAGACAGAAGATGAGTG |
|  | 5’-GTTCGGAGCATTCAACGCTG |
CerS6/Lass6 | 5’-CTGAGTCGTGAAGACAGAGG |
| Â | 5'-CACCGGTACCTCGGAGCGGA |
Des1 | 5'-GTTTGGGATTGATGAACAGGGGT |
Glucosylceramide | 5’-GCTCAGTACATTGCTGAAGA |
Synthase | 5’-AGTACGAACCAGAGTTTTGC |