Three-dimensional structure of β-cell-specific zinc transporter, ZnT-8, predicted from the type 2 diabetes-associated gene variant SLC30A8 R325W

Table 3 Sequence alignment of the loop domain linking β2 to α2 of YiiP and representative members of the cation diffusion facilitator family.

CDF homologs	Amino acid sequence	Residue number
	◀----β2---▶ ◀-L3-▶ ◀----- α2 --------
YiiP	PTRFIQIHLE MEDSLP LVQAHMVADQVEQAI	286
CzrB	PRSFLEFHLV VRGDTP VEEAHRLCDELERAL	269
ZitB	EKPVMTLHVQ VI---P PHDHDALLDQIQHYL	275
CzcD	GKASLTVHVV NDTA-- VNPEMEVLPELKQML	273
msZnT-8	NQVILSVHVA TA---A SQDSQSVRTGIAQAL	337
hmZnT-2	AQPVLSVHIA IAQN-- -TDAQAVLKTASSRL	293
hmZnT-3	TYHVASAHLA IDST-- -ADPEAVLAEASSRL	357
hmZnT-8	NQVILSAHVA TA---A SRDSQVVRREIAKAL	339

The human ZnT-8 residue R325 is in boldface. YiiP and ZitB are bacterial CDF homologs from Escherichia coli, CzcD from Ralstonia metallidurans, CzrB from Thermus thermophilus, msZnT-8 from Mus musculus (mouse), and ZnT-2, ZnT-3, and ZnT-8 from Homo Sapiens.

ISSN: 1758-5996