Table 1 Primer used for qPCR
Gene | Accession number | Nucleotide sequence (from 5′ to 3′) | Amplicon (bp) |
|---|---|---|---|
CYP-7A1 | NM-012942.2 | F:TGGCTGTCATGTGCAGGCGCCAA R:ACGCACTGCCTCAGCAAACACACA | 159 |
CYP7B1 | NM_019138.1 | F: ACCACAGTCGCATGTTTCTGGGCA R: TCCGCTAAGCTTCTCTGCCACCCT | 108 |
TGR5 | NM_177936.1 | F:GCCCGCTGTGGGGGCCACTGCCCT R:GGGTGCATCACGGCACACCGCCCGC | 109 |
β-actin | XM-032887061.1 | F:AAGTCCCTCACCCTCCCAAAAG R: AAGCAATGCTGTCACCTTCCC | 96 |