Fig. 4

High-glucose-stimulated GβL-ubiquitination and mTORC1 assembly in the cultured MFLECs. A The protein levels of TRAF2, OTUD7B, p-mTOR, mTOR, and GβL. B The protein levels of p-Sin1, Sin1, p-Rictor, Rictor, p-Raptor, Raptor, p-Akt and Akt. C The cell lysates were incubated with anti-GβL antibody-coated beads. The mTOR, Raptor, Rictor, Sin1, and ubiquitin levels in the precipitates were determined via western blot analysis (n = 3 in triplicate, **p < 0.01, ***p < 0.001)