Fig. 2

miR-132-3p is downregulated whereas KLF7 is upregulated in aortas of diabetic patients. A Overview of miRs that are downregulated in both the micronome of aortas from db/db mice and glucose-treated human umbilical vein endothelial cells (HUVECs). The values represent LogFC. B qRT-PCR analysis of miR-132-3p expression in aortic tissue from diabetic patients vs control subjects (n = 7). miR-132-3p is downregulated in aortic tissue from diabetic patients. C Overview of the predicted targets of miR-132-3p with the corresponding predicted targeting sites. D qRT-PCR analysis of SMAD2 and ZEB2 expression in diabetic patients vs control subjects (n = 7). SMAD2 remains unchanged and ZEB2 is downregulated in aortic tissue from diabetic patients. E qRT-PCR analysis of KLF7, PTEN, DNMT3a and ZBTB20 in aortic tissue from diabetic patients vs control subjects (n = 7). KLF7 is upregulated in aortic tissue from diabetic patients whereas PTEN, DNMT3a and ZBTB20 remain unchanged. All data is presented as mean value; error bars represent S.E.M.; n.s., not significant; *p < 0.05; **p < 0.01