Fig. 4

Plasma-EVs transfer miR-30b to RMECs and thus induce angiogenesis. A Morphology of the isolated plasma-EVs from control and PDR mice observed under a TEM (100 μm). B The particle size of the isolated plasma-EVs from control and PDR mice measured by nanoparticle tracking analyzer. C Western blot analysis of EV marker proteins CD63, CD81, Alix, and Calnexin in the isolated plasma-EVs from control and PDR mice. D Expression of miR-30b determined by RT-qPCR in the plasma-EVs isolated from control and PDR mice. E Internalization of PKH67-labeled EVs by RMECs (25 μm). F Expression of miR-30b and SIRT1 determined by RT-qPCR in the RMECs co-cultured with plasma-EVs. G Western blot analysis of SIRT1 and VEGF proteins in the RMECs co-cultured with plasma-EVs. H EdU-positive RMECs co-cultured with plasma-EVs (50 μm). I Angiogenesis of RMECs co-cultured with plasma-EVs determined by vessel-like tube formation assay (50 μm). *p < 0.05, compared with the RMECs treated with PBS. **p < 0.01, compared with the RMECs co-cultured with plasma-EVs from control mice. Cell experiments were conducted 3 times independently