Fig. 3From: Effect of plasma-derived extracellular vesicles on angiogenesis and the ensuing proliferative diabetic retinopathy through a miR-30b-dependent mechanism Inhibition of miR-30b alleviates HG-induced angiogenesis through upregulation of SIRT1. RMECs were transduced with In-miR-30b + sh-NC or In-miR-30b + sh-SIRT1. A Expression of miR-30b and SIRT1 determined by RT-qPCR in RMECs. B Western blot analysis of SIRT1 protein in RMECs. C EdU-positive RMECs (50 μm). D Angiogenesis of RMECs determined by vessel-like tube formation assay (50 μm). E Western blot analysis of VEGF protein in RMECs. *p < 0.05, compared with the RMECs transfected with In-NC + sh-NC. #p < 0.05, compared with RMECs transduced with In-miR-30b + sh-NC. Cell experiments were conducted 3 times independentlyBack to article page