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Fig. 1 | Diabetology & Metabolic Syndrome

Fig. 1

From: Alterations of the expression levels of glucose, inflammation, and iron metabolism related miRNAs and their target genes in the hypothalamus of STZ-induced rat diabetes model

Fig. 1

mRNA (A) and Western blot (B, C) analyses of the target genes of glucose metabolism related miRNAs. Real time PCR was carried out using a SYBR Green protocol. β-actin housekeeping gene was used as the internal control for the normalization of the reaction. The relative gene expression of the control hypothalamus samples was regarded as 1. Real time polymerase chain reactions were carried out in triplicate in each independent experiment. For the WB, the same amount of protein from each lysate was used for the analysis. A mRNA expression levels of target genes. B Western blot analysis of the target proteins. C Optical density analysis of the target proteins. The columns represent mean values, error bars show the standard deviation (SD) of three independent determinations (n = 3). Asterisk indicates p < 0.05 compared to the control. HXK-2: hexokinase-2; INSR: insulin receptor; LEPR: leptin receptor; GCK: glucokinase; GLUT4-glucose transporter 4; IGFR: insulin-like growth factor receptor 1; PEPCK: phosphoenolpyruvate carboxykinase; STZ: streptozotocin

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Diabetology & Metabolic Syndrome

ISSN: 1758-5996

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