Fig. 6

Adiponectin/miR-711 regulates myocardial inflammation and apoptosis in diabetic rats. Diabetic rats were injected with adiponectin or miR-711 + adiponectin. A ELISA was used to determine the contents of serum adiponectin. Quantitative real-time polymerase chain reaction was used to detect the expression of miR-711 B and TLR4 mRNA C in the blood of the rats. Western blotting was used to detect TLR4 D and p-NF-κB p65 E in the myocardium of the rats. F The colorimetric method was used to detect the activity of caspase-3 in the myocardium of the rats. G ELISA was performed to detect the concentrations of serum ICAM-1, MCP-1, and IL-1β. H Hematoxylin and eosin staining was used to reveal myocardial structure. I TUNEL assay was used to reveal myocardial apoptosis. n = 3. The data were all measurement data and expressed as mean ± standard deviation. One-way analysis of variance was used for comparisons between multiple groups and Tukey’s test for post-hoc multiple comparisons. ^* indicates P < 0.05. C control group, D diabetes group, DA diabetes + adiponectin group, DMA diabetes + miR-711 inhibitor + adiponectin group, TLR4 toll-like receptor 4, ICAM intercellular adhesion molecule, MCP monocyte chemotactic protein, IL interleukin, TUNEL terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling