Fig. 4

miR-711 affects the biological behaviors of high glucose-treated H9c2 cells by regulating TLR4/NF-κB. High glucose-treated H9c2 cells were transfected with mimic-NC, miR-711 mimic, inhibitor-NC or miR-711 inhibitor. A MTT assay was performed to assess cell viability. B β-galactosidase staining was used to reveal cell senescence. C Flow cytometry was used to analyze cell apoptosis. D The colorimetric method was used to detect the activity of caspase-3 in cells. E DCFH-DA probes were used to detect ROS in cells. F Western blotting was used to detect Bax and Bcl-2 in cells. The data were all measurement data and expressed as mean ± standard deviation. One-way analysis of variance was used for comparisons between multiple groups and Tukey's test for post-hoc multiple comparisons. ^* indicates P < 0.05. The experiments were repeated thrice. HG high glucose, NC negative control, TLR4 toll-like receptor 4, NF-κB nuclear factor κB, MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; DCFH-DA, 2′,7′-dichlorodihydrofluorescein diacetate; ROS reactive oxygen species, Bax Bcl-2-associated X protein; Bcl-2 B-cell lymphoma-2