Fig. 1

Adiponectin and miR-711 were underexpressed in high glucose-treated H9c2 cells. H9c2 cells were treated with mannitol or high glucose. A MTT was used to detect cell viability. B qRT-PCR was used to detect the expression of adiponectin in cells. C ELISA was performed to detect the expression of adiponectin in cell culture supernatant. D qRT-PCR was used to detect the expression of miR-711 in cells. E ELISA was performed to detect the expression of ICAM-1, MCP-1, and IL-1β in cell culture supernatant. The data were all measurement data and expressed as mean ± standard deviation. One-way analysis of variance was used for comparisons between multiple groups and Tukey's test for post-hoc multiple comparisons. * indicates P < 0.05. The experiments were repeated thrice. OS osmotic, HG high glucose, MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, qRT-PCR quantitative real-time polymerase chain reaction, ELISA enzyme-linked immunosorbent assay, ICAM intercellular adhesion molecule, MCP monocyte chemotactic protein, IL interleukin