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Fig. 3 | Diabetology & Metabolic Syndrome

Fig. 3

From: MiR-574-3p inhibits glucose toxicity-induced pancreatic β-cell dysfunction by suppressing PRMT1

Fig. 3

PRMT1 silencing attenuates the β-cell dysfunction caused by high glucose treatment. A The efficiencies of siRNA of PRMT1 examined by RT-qPCR. B CCK-8 assay assessing cell proliferation of MIN6 cells in presence or absence of a high glucose concentration. MIN6 cells transfected with si-PRMT1 or si-NC were cultured for 24 h in high glucose conditions. C Cell apoptosis was determined by flow cytometry. D Cell AGE levels were determined using an AGEs Assay kit. E Production of ROS was detected utilizing CM-DCF fluorescence. F ELISA assessed glucose-stimulated insulin secretion in the presence or absence of high glucose conditions. G GLUT1 level was examined by RT-qPCR. MIN6 cells transfected with si-PRMT1 or si-NC were cultured for 24 h in high glucose conditions. **P < 0.01

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