Fig. 2
From: MiR-574-3p inhibits glucose toxicity-induced pancreatic β-cell dysfunction by suppressing PRMT1
Gluco-toxicity induced the PRMT1 overexpression. A MIN6 cells were exposed to 25 mM glucose for 6, 12, and 24 h. CCK8 assays were performed to detect the proliferation. B MIN6 cells were exposed to 5.5, 16.7, 25, and 33.3 mM glucose for 2 h. CCK8 assays were performed to detect the proliferation. C RT-qPCR was used to determine fold changes in expression of PRMT1 in MIN6 cells exposed to 25 mM glucose for 6, 12, and 24 h. D Western blotting was used to determine fold changes in the expression of PRMT1 in MIN6 cells exposed to 25 mM glucose for 6, 12, and 24 h. E Immunofluorescence assay was used to determine PRMT1 fluorescence intensity in MIN6 cells exposed to 25 mM glucose for 6, 12, and 24 h. F RT-qPCR was used to determine fold changes in expression of PRMT1 in MIN6 cells exposed to 5.5, 16.7, 25, and 33.3 mM glucose for 2 h. G Western blotting was used to determine fold changes in the expression of PRMT1 in MIN6 cells exposed to 5.5, 16.7, 25, and 33.3 mM glucose for 2 h. H Immunofluorescence assay was used to determine the PRMT1 fluorescence intensity in MIN6 cells exposed to 5.5, 16.7, 25, and 33.3 mM glucose for 2 h. **P < 0.01 vs. NC