Fig. 8

Photomicrograph (magnification ×20) of the hepatic parenchyma stained with hematoxylin–eosin (HE) of non-obese male (A–C) and obese (D–F) Wistar rats after 28 days under treatment by gavage with placebo (propylene glycol), CN (0.2 mg/kg/day CN for 8 days and 0.4 mg/kg/day for the subsequent 20 days) or 2CN (0.4 mg/kg/day CN for 8 days and 0.8 mg/kg/day for the subsequent 20 days). A G1: normal hepatic parenchyma. B G2: discrete mononuclear infiltrate in the portal space (arrow), without degenerative or necrotic lesions in hepatocytes. C G3: normal hepatocytes. D G4: hepatocytes with moderate diffuse cytoplasmic vacuolation (arrows). E G5: some hepatocytes with mild cytoplasmic vacuolation (arrows). F G6: mild mononuclear infiltrate (arrow) and rare hepatocytes with cytoplasmic vacuolation (HE)