Table 1 Sequences of primer pairs used for amplification of mRNA by real time PCR
Target | Forward primer | Reverse primer |
|---|---|---|
FGFR2 | GCCTCTCGAACAGTATTCTCCT | ACAGGGTTCATAAGGCATGGG |
FGFR3 | TGGATCAGTGAGAATGTGGAGG | CCTATGAAATTGGTGGCTCGAC |
FGFR4 | GCTCGGAGGTAGAGGTCTTGT | CCACGCTGACTGGTAGGAA |
SREBP1 | ACTTCTGGAGACATCGCAAAC | GGTAGACAACAGCCGCATC |
ACC1 | TGGTCGTGACTGCTCTGTGC | GTAGCCGAGGGTTCAGTTCC |
SCD1 | ATGTGCCAGAGGAGCTGAGT | TGATCCACTGTTGCTTCTGC |
Actin | CGTAAAGACCTCTATGCCAACA | CGGACTCATCGTACTCCTGCT |
FAS | GCTGCGGAAACTTCAGGAAAT | AGAGACGTGTCACTCCTGGACTT |
AMPK | TACTCAACCGGCGAG CG | AGACGGCGGCTTTCCTTTT |
PPARα | AGGAAGCCGTTCTGTGACAT | TTGAAGGAGCTTTGGGAAGA |
LKB1 | TTCAACCGAGTGGGTTATTTTGT | TACTGGGCATCTGGGGTGTAG |
Actin | CGTAAAGACCTCTATGCCAACA | CGGACTCATCGTACTCCTGCT |