Fig. 4

FSH activates Erk, Akt, and CREB via Gsα to produce rennin in As4.1 cells. A Western blotting results shows Gsα isoforms is expressed in As4.1 cells. B Effect of FSH and forskolin (Forskol, 10 mM) on cAMP levels in As4.1 cells cultures. Data are presented as mean ± SEM; n = 5; *P < 0.05, **P < 0.01, ***P < 0.001, compared with the 0ng/ml FSH treatment. P-values were 0.03228, 0.00574, 0.00072 respectively. C–F Western blots of phosphorylated (p-) Erk1/2, Akt, CREB, JNK, or p38 levels or nuclear c-Fos levels in As4.1 cells in response to FSH. Data are presented as mean ± SEM; n = 5; *P < 0.05, **P < 0.01, ***P < 0.001, compared with the 0ng/ml FSH treatment. P-values for panel C were 0.0069, 0.0297, respectively. P-values for panel D were 0.00533, 0.00229, 0.00076, respectively. P-values for panel E were 0.00178, 0.00043, respectively. P-values for panel F were 0.03985, 0.00085, respectively. G Effect of FSH on rennin concentration in the supernatant of As4.1 cells cultures after Gsα was inhibited by NF449 (100ng/mL). Data are presented as mean ± SEM; n = 5; *P < 0.05, **P < 0.01, compared with the 0ng/ml FSH treatment. P-values were 0.0414, 0.0075, respectively. H Effect of PKA inhibitors H89 (30 µM) and MEK1/2 inhibitors U0126 (0.5 mM) on rennin concentration in the supernatant of As4.1 cells cultures in response to 30 ng/ml FSH treatment. Data are presented as mean ± SEM; n = 5; **P < 0.01, compared with the corresponding controls. P-values were 0.0082, 0.0061, 0.0047, respectively. I Quantitative real-time PCR showing the effect of the MEK1/2 inhibitors U0126 (0.5 mM) or JNK inhibitors SB600125 (0.5 mM) on rennin mRNA expression in 48-hour cultures. Data are presented as mean ± SEM; n = 5; *P < 0.05, **P < 0.01, ***P < 0.001, compared with the corresponding controls. P-values were 0.0056, 0.0309, respectively