Fig. 2

FSH Stimulates As4.1 cells to produce rennin. A Quantitative real-time PCR shows the effect of FSH or LH (100 ng/ml) on rennin mRNA expression in 2-day cultures from As4.1 cells. Data are presented as mean ± SEM; n = 5; *P < 0.05, **P < 0.01, compared with the 0ng/ml FSH treatment. P-values were 0.0073, 0.0386, respectively. B The in vitro culture shows the effect of FSH or LH (100 ng/ml) on the production of renin as the supernatant renin concentration in As4.1 cells. Data are presented as mean ± SEM; n = 5; *P < 0.05, **P < 0.01, compared with the 0ng/ml FSH treatment. P-values were 0.0067, 0.0413, respectively. C FSH has no effect on the proliferation of As4.1 cells. Mean absorbance (Abs, ±SEM) at 490 nm. Data are presented as mean ± SEM; n = 5; compared with the 0ng/ml FSH treatment. D Effect of FSH on rennin mRNA expression in As4.1 cells after FSHR knockdown by siRNA transfection using quantitative real-time PCR. Data are presented as mean ± SEM; n = 5; *P < 0.05, **P < 0.01, compared with the corresponding controls. P-values were 0.0047, 0.0334, respectively. E Effect of FSH on rennin mRNA expression in scrambled RNA trasfection (control), FSHR siRNA trasfection or TSHR siRNA trasfection in As4.1 cells. Data are presented as mean ± SEM; n = 5; **P < 0.01, compared with the corresponding controls. P-values were 0.0068, 0.0052, respectively. F Effects of FSH on rennin concentration in supernatant of medium by invitro culture (Fa) and rennin mRNA by quantitative real-time PCR (Fb) in As4.1 cells infected with adenovirus containing the mouse FSHR gene or empty vector. Data are presented as mean ± SEM. n = 5; *P < 0.05, **P < 0.01, ***P < 0.001, compared with the corresponding controls. P-values for panel Fa were 0.04065, 0.00776, 0.00083, respectively. P-values for panel Fb was 0.0038