Fig. 6

Effects of miR-147a and WNT2B on the proliferation, fibrosis and oxidative stress of HG-induced HMC. A The transfection efficiency of miR-147a mimic was determined by measuring miR-147a expression using qRT-PCR. B–C QRT-PCR and WB analysis were used to detect WNT2B mRNA and protein expression to evaluate the transfection efficiency of pcDNA-WNT2B. D–E HMC was transfected with mimic NC, miR-147a mimic, miR-147a mimic + pcDNA or miR-147a mimic + pcDNA-WNT2B. The mRNA and protein expression of WNT2B was examined by qRT-PCR and WB analysis. F–O Transfected HMC was treated with HG. F CCK8 assay was employed to test HMC viability. G–H The percentage of EDU positive cells was assessed using EDU staining. I–L WB analysis was performed to detect he protein levels of PCNA, CyclinD1, fibronectin, collagen I and α-SMA. M–O Corresponding Assay Kits were utilized to assess the ROS level, SOD activity and MDA level. *P < 0.05