Fig. 4

Effects of lncRNA HOTAIR silencing and miR-147a inhibitor on the biological functions of HG-induced HMC. A The transfection efficiency of miR-147a inhibitor was confirmed by detecting miR-147a expression using qRT-PCR. B HMC was transfected with si-NC, si-lncRNA HOTAIR, si-lncRNA HOTAIR + inhibitor NC or si-lncRNA HOTAIR + miR-147a inhibitor. The expression of miR-147a was measured by qRT-PCR. C–L Transfected HMC was treated with HG. C CCK8 assay was performed to detect HMC viability. D–E EDU staining was used to examine the percentage of EDU positive cells. F–I WB analysis was utilized for measuring the protein levels of PCNA, CyclinD1, fibronectin, collagen I and α-SMA. J–L The ROS level, SOD activity and MDA level were analyzed using corresponding Assay Kits. *P < 0.05