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Fig. 2 | Diabetology & Metabolic Syndrome

Fig. 2

From: LncRNA HOTAIR facilitates high glucose-induced mesangial cell proliferation, fibrosis and oxidative stress in diabetic nephropathy via regulating miR-147a/WNT2B axis

Fig. 2

Effects of lncRNA HOTAIR silencing on the biological functions of HG-induced HMC. A LncRNA HOTAIR expression was detected by qRT-PCR in HMC treated with HG or NG. B QRT-PCR was used to measure lncRNA HOTAIR expression in HMC transfected with si-NC or sh-lncRNA HOTAIR. C–L Transfected HMC was treated with HG. C HMC viability was assessed by CCK8 assay. D–E The percentage of EDU positive cells was evaluated by EDU staining. F–I The protein levels of PCNA, CyclinD1, fibronectin, collagen I and α-SMA were examined using WB analysis. J–L Corresponding Assay Kits were used to determine the ROS level, SOD activity and MDA level. *P < 0.05

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Diabetology & Metabolic Syndrome

ISSN: 1758-5996

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