Fig. 5From: Trigonelline reverses high glucose-induced proliferation, fibrosis of mesangial cells via modulation of Wnt signaling pathwayExpression of β-catenin, TCF4, Wnt5a, Wnt4, and GSK-3β in HMCs. After treatment, proteins were extracted from the cells and assessed by western blot analysis. GAPDH served as the loading control. A Representative western blot of β-catenin, TCF4, Wnt5a, Wnt4, and GSK-3β expression. B The relative β-catenin, TCF4, and Wnt5a protein expression detected by western blot. NG normal glucose, HG high glucose, A TRL + high glucose, B TRL + β-catenin siRNA + high glucose, C β-catenin pcDNA + high glucose, D TRL + β-catenin pcDNA + high glucose, E ICG-001 + high glucose; #P < 0.05 versus NG, ##P < 0.01 versus NG; *P < 0.05 versus HG, **P < 0.01 versus HG; △P < 0.01 versus group A, △△P < 0.01 versus group A; ▲P < 0.05 versus group C, ▲▲P < 0.01 versus group CBack to article page