Fig. 4

Effects of TRL on β-catenin, Wnt5a, and TCF4 expression in HMCs. HMCs were treated with various concentrations of TRL (50, 100, or 200 μM) for 24 h. Proteins were extracted from cells and assessed by western blot analysis. Total RNA was extracted from treated cells and assessed by qRT-PCR analysis. GAPDH served as the loading control. A Representative western blots of β-catenin, TCF4, Wnt5a, Wnt4, and GSK-3β expression. B β-catenin, TCF4, Wnt5a, Wnt4, and GSK-3β protein expression were detected by western blot. C Immunohistochemical staining (×200) of β-catenin, Wnt5a, and TCF4. NG normal glucose, HG high glucose, TRL (50, 100, 200 μM): TRL + high glucose; ^#P < 0.05 versus NG, ^##P < 0.01 versus NG; *P < 0.05 versus HG, **P < 0.01 versus HG