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Fig. 3 | Diabetology & Metabolic Syndrome

Fig. 3

From: LncRNA ZFAS1 regulates the proliferation, oxidative stress, fibrosis, and inflammation of high glucose-induced human mesangial cells via the miR-588/ROCK1 axis

Fig. 3

ZFAS1 endogenously targets the miR-588 sequence. Bioinformatics prediction of target miRNA. Lncbase predicted that the ZFAS1 targets miR-588 seed region sequences. Dual-luciferase reporter assay experiment. MiR-588 mimics significantly reduced the luciferase activity of HGMC co-transfected with the ZFAS1 wild type (WT) reporter plasmid but had no inhibitory effect on that of ZFAS1 mutant type (Mut) plasmid (A). Biotinylated RNA pull-down assay. Biotin-coupled probes with wild type (WT) miR-588 sequence pull-down more ZFAS1 than those with the mutated miR-588 sequence (MUT) (B). RIP-qRT-PCR analysis. More ZFAS1 and miR-588 were significantly enriched in the immunoprecipitated Ago2-containing complexes compared to the IgG group (C). Experiments were carried out in triplicates and p < 0.05 was chosen as the significance level (*** p < 0.001 and ###p < 0.001)

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