Fig. 5

Overexpression of FGD5-AS1 boosted apoptosis and alleviated growth and calcification, of high glucose-induced HA-VSMC cells. A The level of FGD5-AS1 was confirmed through RT-qPCR in different groups. B CCK-8 assay showed that upregulation of FGD5-AS1 reversed the increase of cell proliferation in HA-VSMCs caused by high glucose. C Upregulation of FGD5-AS1 partly offset the change of apoptosis markers, caspase-3, Bcl-2 and Bax, caused by high glucose. D, E Upregulation of FGD5-AS1 reduced the increased level of calcification marker, alkaline phosphatase and osteocalcin. *P  < 0.05, **P  < 0.01, ***P  < 0.001 (compared with mock). ^#P  < 0.05, ^##P  < 0.01, ^###P  < 0.001 (compared with HG  +  ov-NC). HG high glucose; ALP alkaline phosphatase