Fig. 4
From: TLR9 regulates NLRP3 inflammasome activation via the NF-kB signaling pathway in diabetic nephropathy
Role of TLR9 knockdown and BA treatment in the NF-kB-NLRP3 inflammasome pathway of HG-induced MCs. HG-treated MCs were transfected with shRNA-TLR9 or shRNA-TLR9 for 36 h and then treated the 1 µm for twelve hours. A RT-PCR was conducted to determine the mRNA expression level of TLR9 in MCs. B WB was conducted to determine the protein levels of TLR9, NF-kB, NLRP3, nuclear NF-kB, phosphor NF-kB, PYCARD, interleukin-1β, and cysteinyl aspartate-specific proteinase-1 in MCs. The data are representative of the results of three independent experiments, and the data are presented as means ± S.E.M (**P < 0.01, ***P < 0.001)