Fig. 2
From: TLR9 regulates NLRP3 inflammasome activation via the NF-kB signaling pathway in diabetic nephropathy
Effect of TLR9 knockdown on the NF-kB-NLRP3 inflammasome pathway in renal tissues of experimental mice (db/db). Experimental mice (db/db) were administered lentiviral-shRNA-NC or lentiviral-shRNA-TLR9 via tail vein injection. A Representative IHC image of TLR9, phosphor NF-kB, and NLRP3 antibodies in the glomeruli of mice (db/m) and mice with DN (db/db). B WB was conducted to determine the protein levels of TLR9, NF-kB, NLRP3, nuclear NF-kB, phosphor NF-kB, PYCARD, IL-1β, and cysteinyl aspartate-specific proteinase-1 in the renal tissues of mice (db/m) and mice with DN (db/db)