Fig. 1
From: TLR9 regulates NLRP3 inflammasome activation via the NF-kB signaling pathway in diabetic nephropathy
Expression of TLR9 in DN. A Real-time PCR revealed TLR9 expression in PBMCs from patients (healthy = 7 and DN patients = 17). B WB revealed protein levels of TLR9 in PBMCs from patients (healthy = 3 and DN patients = 3). C Real-time PCR revealed TLR9 mRNA expression in the kidneys of mice (db/db) with DN (n = 6) and mice (db/m) (n = 6) at 12 weeks. D WB revealed TLR9 protein levels in the kidneys of mice (db/db) with DN and control mice (db/m) at 12 weeks. E MCs were treated with HG for more than 12 h. RT-PCR was used to determine the level of TLR9 mRNA expression in the MCs treated with HG or normal LG. F WB was used to determine the protein levels of TLR9 in the MCs treated with HG or normal LG. The data are representative of the results of three independent experiments, and the data are presented as means ± S.E.M (*P < 0.05, **P < 0.01, ***P < 0.001)