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Fig. 4 | Diabetology & Metabolic Syndrome

Fig. 4

From: Histone lysine-specific demethylase 1 induced renal fibrosis via decreasing sirtuin 3 expression and activating TGF-β1/Smad3 pathway in diabetic nephropathy

Fig. 4

Knockdown of LSD1 suppresses NRK-49F cells activation. The NRK-49F cells were transfected with Ad-shNC or Ad-shLSD1 and then treated with 10 ng/ml TGF-β1 for 72 h. A The expression and location of α-SMA were detected by IF assay (bar = 100 μm). B The protein levels of LSD1, α-SMA, Fibronectin, collagen I and collagen IV were evaluated by western blot. Each experiment repeated three times. **p < 0.01 vs. Control. ##p < 0.01 vs. TGF-β1 + Ad-shLSD1

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Diabetology & Metabolic Syndrome

ISSN: 1758-5996

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