Fig. 5
From: MiR-423-5p activated by E2F1 promotes neovascularization in diabetic retinopathy by targeting HIPK2
MiR-423-5p targeted and negatively regulated HIPK2 expression. A Complementary binding sites between miR-423-5p and HIPK2 mRNA. B miR-423-5p mimics diminished luciferase activity of HIPK2-WT but did not change the activity of HIPK2-MUT. C miR-423-5p mimics increased miR-423-5p level but reduced HIPK2 mRNA level in the cells while miR-423-5p inhibitor had opposite effects. D miR-423-5p mimics diminished HIPK2 protein level while miR-423-5p inhibitor increased its level. All results were presented as the mean ± SD (n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001. WT, wild-type; MUT, mutant; NC, negative control; HIPK2, homeodomain-interacting protein kinase 2