Circ-FBXW12 aggravates the development of diabetic nephropathy by binding to miR-31-5p to induce LIN28B

Table 2 Primers sequences used for qRT-PCR

Primers	Sequences (5′–3′)	Tm	PCR product	Ct range
circ-FBXW12-forward	ACACGTGGCATGATCACACA	60.25	125	0.8
circ-FBXW12-reverse	ACTCCTGGACTGGGCTTGAC	58.4	125	0.8
FBXW12-forward	CTGCCTGGGTTAAGAGATGTTT	58.31	115	1.2
FBXW12-reverse	AGGTACGACTGTATGTCCCAC	58.9	115	1.2
miR-31-5p-forward	ACACTCCAGCTGGGAGGCAAGATGCTGGC	62.43	69	1.7
miR-31-5p-reverse	TGGTGTCGTGGAGTCG	55.46	69	1.7
LIN28B-forward	TTGAGTCAATACGGGTAACAGGA	59.17	184	0.9
LIN28B-reverse	TGACAGTAATGGCACTTCTTTGG	59.18	184	0.9
GAPDH-forward	ACAGTCAGCCGCATCTTCTT	59.68	94	1.8
GAPDH-reverse	ACGACCAAATCCGTTGACTC	58.21	94	1.8
U6-forward	CCTCGCTTCGGCAGCACATA	62.91	94	1.3
U6-reverse	ACGCTTCACGAATTTGCGT	59.06	94	1.3

ISSN: 1758-5996