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Fig. 6 | Diabetology & Metabolic Syndrome

Fig. 6

From: Circ-FBXW12 aggravates the development of diabetic nephropathy by binding to miR-31-5p to induce LIN28B

Fig. 6

LIN28B knockdown suppressed cell viability, inflammation, cell cycle process, ECM production and oxidative stress in HG-treated HMCs. HMCs were transfected with si-con or si-LIN28B under HG condition. A The protein level of LIN28B in HMCs was measured via western blot assay. B The viability of HMCs was evaluated by CCK-8 assay. C The levels of IL-6 and TNF-α in HMCs were determined by ELISA kits. D The cell cycle process in HMCs was analyzed by flow cytometry analysis. E The proliferation of HMCs was tested by EdU assay. F–H The protein levels of CyclinD1, P21, collagen I, collagen IV and TGF-β1 in HMCs were measured via western blot assay. I and J The activity of SOD and the level of MDA in HMCs were measured by relevant kits. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

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