Fig. 4

Circ-FBXW12 regulated cell proliferation, inflammation, cell cycle, ECM production and oxidative stress in HG-treated HMCs by targeting miR-31-5p. A The expression of miR-31-5p in HMCs transfected with anti-miR-NC or anti-miR-31-5p was determined by RT-qPCR assay. (B-I) HMCs cells were transfected with si-NC, si-circ-FBXW12, si-circ-FBXW12 + anti-miR-NC or si-circ-FBXW12 + anti-miR-31-5p under HG condition. B The expression of miR-31-5p in HMCs was detected by RT-qPCR assay. C HMC viability was assessed by CCK-8 assay. D The concentrations of IL-6 and TNF-α in HMCs were examined with ELISA kits. E The cell cycle process in HMCs was analyzed by flow cytometry analysis. F The proliferation of HMCs was assessed by EdU assay. G–I The protein levels of CyclinD1, P21, collagen I, collagen IV and TGF-β1 in HMCs were measured via western blot assay. J and K SOD activity and MDA level in HMCs were detected with specific kits. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001