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Fig. 3 | Diabetology & Metabolic Syndrome

Fig. 3

From: Circ-FBXW12 aggravates the development of diabetic nephropathy by binding to miR-31-5p to induce LIN28B

Fig. 3

Circ-FBXW12 directly interacted with miR-31-5p. A The binding sites between circ-FBXW12 and miR-31-5p. B The expression of miR-31-5p in HMCs transfected with miR-NC or miR-31-5p was detected by RT-qPCR assay. C and D The interaction between miR-31-5p and circ-FBXW12 was demonstrated by dual-luciferase reporter assay and RIP assay. E and F The expression of miR-31-5p in DM, DN patients’ serums and HG-stimulated HMCs was determined through RT-qPCR assay. G The linear correlation between the levels of circ-FBXW12 and miR-31-5p in the serums of DN patients was analyzed by Spearman’s correlation coefficient analysis. H The expression of circ-FBXW12 in HMCs transfected with pCD5-ciR or circ-FBXW12 in HG condition was detected by RT-qPCR assay. I After HMCs were transfected with si-NC, si-circ-FBXW12, pCD5-ciR or circ-FBXW12 in HG condition, the expression of miR-31-5p was detected by RT-qPCR assay. ***P < 0.001, ****P < 0.0001

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Diabetology & Metabolic Syndrome

ISSN: 1758-5996

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