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Fig. 2 | Diabetology & Metabolic Syndrome

Fig. 2

From: Circ-FBXW12 aggravates the development of diabetic nephropathy by binding to miR-31-5p to induce LIN28B

Fig. 2

Circ-FBXW12 silencing reversed HG-mediated effects on cell growth, inflammation, cell cycle, ECM production and oxidative stress in HMCs. HMCs were assigned to Control, HG, HG + si-NC and HG + si-circ-FBXW12 groups. A The expression of circ-FBXW12 in HMCs was determined by RT-qPCR assay. B The viability of HMCs was assessed by CCK-8 assay. C The concentrations of IL-6 and TNF-α in HMCs were examined with ELISA kits. D The cell cycle process in HMCs was analyzed by flow cytometry analysis. E The proliferation of HMCs was evaluated by EdU assay. F–H The protein levels of CyclinD1, P21, collagen I, collagen IV and TGF-β1 in HMCs were measured via western blot assay. I and J The activity of SOD and the level of MDA in HMCs were examined with specific commercial kits. **P < 0.01, ***P < 0.001, ****P < 0.0001

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Diabetology & Metabolic Syndrome

ISSN: 1758-5996

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