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Fig. 7 | Diabetology & Metabolic Syndrome

Fig. 7

From: CircSMAD4 alleviates high glucose-induced inflammation, extracellular matrix deposition and apoptosis in mouse glomerulus mesangial cells by relieving miR-377-3p-mediated BMP7 inhibition

Fig. 7

MiR-377-3p overexpression aggravated HG-induced SV40-MES13 cell inflammation, ECM deposition and cell apoptosis by suppressing BMP7. A The efficiency of BMP7 overexpression was tested by western blot. BI HG-treated SV40-MES13 cells were transfected with miR-377-3p or miR-377-3p + BMP7. B The expression of BMP7 protein was detected by western blot. CF The releases of IFN-γ, MCP-1, IL-6 and TNF-α in medium were determined by ELISA. G The protein levels of fibronectin and collagen IV were measured by western blot. H Cell apoptosis was assessed by flow cytometry assay. I The protein levels of Bcl-2 and Bax were measured by western blot. *P < 0.05

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