Fig. 6
From: LncRNA KCNQ1OT1 promotes the development of diabetic nephropathy by regulating miR-93-5p/ROCK2 axis
MiR-93-5p regulated proliferation, apoptosis and fibrosis by targeting ROCK2 in HG-stimulated HGMC and HRGEC. HGMC and HRGEC were transfected with miR-NC, miR-93-5p, miR-93-5p + pcDNA-con, or miR-93-5p + pcDNA-ROCK2, followed by treatment with HG. A and B Cell proliferation was assessed using CCK-8 assay. C Cell apoptosis was analyzed via flow cytometry analysis. D and E Western blot assay was conducted to examine the protein expression of Bax and Bcl-2. F–I FN, Col-I, Col-IV, E-Cadherin, vimentin, and N-Cadherin protein levels were measured by western blot assay. *P < 0.05