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Fig. 5 | Diabetology & Metabolic Syndrome

Fig. 5

From: LncRNA KCNQ1OT1 promotes the development of diabetic nephropathy by regulating miR-93-5p/ROCK2 axis

Fig. 5

ROCK2 could directly bind to miR-93-5p. A TargetScan predicted the binding sites of ROCK2 and miR-93-5p. B–E Dual-luciferase reporter and RIP assays were performed in HGMC and HRGEC to validate the association between ROCK2 and miR-93-5p. F ROCK2 mRNA expression in the serum samples of DN patients was examine by qRT-PCR. G The correlation between ROCK2 mRNA expression and miR-93-5p expression was analyzed in serum samples of DN patients. H ROCK2 protein expression was detected by western blot assay in HGMC and HRGEC transfected with pcDNA-con or pcDNA-ROCK2. I and J The protein expression of ROCK2 was tested by western blot assay in HGMC and HRGEC transfected with miR-NC, miR-93-5p, miR-93-5p + pcDNA-con, or miR-93-5p + pcDNA-ROCK2. *P < 0.05

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