Fig. 3
From: LncRNA KCNQ1OT1 promotes the development of diabetic nephropathy by regulating miR-93-5p/ROCK2 axis
MiR-93-5p was a target of KCNQ1OT1. A The complementary binding sites between KCNQ1OT1 and miR-93-5p were predicted by starBase. B The expression of miR-93-5p was detected by qRT-PCR in HGMC and HRGEC transfected with miR-93-5p or miR-NC. C and D Dual-luciferase reporter assay was conducted to measure the luciferase activity in HGMC and HRGEC co-transfected with KCNQ1OT1-WT or KCNQ1OT1-MUT and miR-NC or miR-93-5p. E and F RIP assay was performed in HGMC and HRGEC and enrichment of KCNQ1OT1 and miR-93-5p was detected by qRT-PCR. G The expression of miR-93-5p was measured using qRT-PCR in HGMC and HRGEC transfected with anti-miR-NC or anti-miR-93-5p. H and I The expression of miR-93-5p was determined by qRT-PCR in HGMC and HRGEC transfected with si-NC, si-KCNQ1OT1, si-KCNQ1OT1 + anti-miR-NC, or si-KCNQ1OT1 + anti-miR-93-5p. *P < 0.05