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Fig. 5 | Diabetology & Metabolic Syndrome

Fig. 5

From: CDKN2B-AS1 participates in high glucose-induced apoptosis and fibrosis via NOTCH2 through functioning as a miR-98-5p decoy in human podocytes and renal tubular cells

Fig. 5

NOTCH2 was a target of miR-98-5p. A The binding sites of NOTCH2 in miR-98-5p were predicted by the starBase database. B, C The luciferase intensity of a luciferase reporter with WT-NOTCH2-3ʹUTR or MUT-NOTCH21-3ʹUTR in HPCs and HK-2 cells transfected with miR-98-5p mimic or miRNA NC was determined through dual-luciferase reporter assay. D The protein level of NOTCH2 in HPCs and HK-2 cells transfected with si-NC or si-NOTCH2 was detected with western blot analysis. E, F Effect of NOTCH2 knockdown on the level of NOTCH2 protein in miR-98-5p-inhibiting HPCs and HK-2 cells was analyzed through western blot analysis. G, H The protein level of NOTCH2 in the serum of DN patients and high glucose-treated HPCs and HK-2 cells was assessed via western blot analysis

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