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Fig. 5 | Diabetology & Metabolic Syndrome

Fig. 5

From: Activation of mTOR mediates hyperglycemia-induced renal glomerular endothelial hyperpermeability via the RhoA/ROCK/pMLC signaling pathway

Fig. 5

HG increased RhoA expression and activity in an mTOR-dependent manner. a HRGECs were treated with rapamycin for 2 h and then stimulated with HG or NG for 22 h. GTP-RhoA levels were measured via pull-down assay. RhoA expression was analyzed via immunoblotting. b RhoA knockdown or si-control–treated HRGECs were incubated with NG or HG for 24 h. RhoA expression and MLC phosphorylation levels were measured using western blotting. After cells were treated with si-RhoA or si-control and stimulated with HG or NG for 24 h, monolayer permeability was assessed via TEER (c) and HRP-albumin leakage permeability assay (d). HG, high glucose; HRGECs, human renal glomerular cells; NG, normal glucose; MLC, myosin light chain; TEER, transendothelial electrical resistance; HRP, horseradish peroxidase. * indicates p < 0.05 vs. the NG + si-Control group. # indicates p < 0.05 vs. the HG + si-Control group

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