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Fig. 3 | Diabetology & Metabolic Syndrome

Fig. 3

From: Activation of mTOR mediates hyperglycemia-induced renal glomerular endothelial hyperpermeability via the RhoA/ROCK/pMLC signaling pathway

Fig. 3

Rapamycin suppressed HG-induced barrier dysfunction by downregulating MLC phosphorylation. a Rapamycin (100 nM) pretreatment for 2 h, HRGECs were incubated with NG or HG for 22 h. F-actin stress fiber formation was assessed via FITC-Phalloidin staining. Cells were stained for F-actin (green) and DAPI (blue) and then imaged. b The intercellular gap area in each group was measured; n = 4. c The levels of MLC phosphorylation were analyzed using western blotting. HG, high glucose; MLC, myosin light chain; HRGECs; human renal glomerular endothelial cells; NG; normal glucose. * indicates p < 0.05 vs. the NG group. # indicates p < 0.05 vs. the HG group

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Diabetology & Metabolic Syndrome

ISSN: 1758-5996

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