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Fig. 5 | Diabetology & Metabolic Syndrome

Fig. 5

From: Long non-coding RNA CASC2 restrains high glucose-induced proliferation, inflammation and fibrosis in human glomerular mesangial cells through mediating miR-135a-5p/TIMP3 axis and JNK signaling

Fig. 5

miR-135a-5p knockdown suppresses HG-induced effects in HMCs partly by up-regulating TIMP3. A The level of miR-135a-5p in HMCs transfected with inhibitor NC or miR-135a-5p inhibitor was determined by RT-qPCR. B The silencing efficiency of si-TIMP3 in HMCs was analyzed by Western blot assay. C HMCs were transfected with inhibitor NC, miR-135a-5p inhibitor, miR-135a-5p inhibitor + si-NC or miR-135a-5p inhibitor + si-TIMP3. Western blot assay was employed to assess the protein expression of TIMP3 in transfected HMCs. D–M HMCs transfected with inhibitor NC, miR-135a-5p inhibitor, miR-135a-5p inhibitor + si-NC or miR-135a-5p inhibitor + si-TIMP3 were induced by HG. D–F CCK8 assay and EDU assay were utilized to analyze cell proliferation ability. G, H Western blot assay was employed to detect the levels of PCNA and CyclinD1 in HMCs. I–K The levels of inflammatory cytokines (Mcp-1, TNF-α and IL-6) were analyzed by ELISA. L, M Western blot assay was utilized to analyze the expression of fibrosis-associated proteins in HMCs. *P < 0.05

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Diabetology & Metabolic Syndrome

ISSN: 1758-5996

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