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Fig. 4 | Diabetology & Metabolic Syndrome

Fig. 4

From: Long non-coding RNA CASC2 restrains high glucose-induced proliferation, inflammation and fibrosis in human glomerular mesangial cells through mediating miR-135a-5p/TIMP3 axis and JNK signaling

Fig. 4

miR-135a-5p binds to the 3ʹUTR of TIMP3. A The potential interaction between miR-135a-5p and TIMP3 was predicted by StarBase database. B The 3ʹUTR fragment of TIMP3, containing the wt or mut putative binding sites with miR-135a-5p, was inserted downstream of the Firefly luciferase gene in luciferase vector to obtain TIMP3 3ʹUTR wt and TIMP3 3ʹUTR mut. C The target relation between miR-135a-5p and TIMP3 in 293T cells was tested by dual-luciferase reporter assay. D, E The protein level of TIMP3 was determined in HMCs induced by NG or HG for 0 h, 12 h, 24 or 48 h by Western blot assay. F The protein level of TIMP3 was measured in HMCs transfected with vector, oe-lncRNA CASC2, oe-lncRNA CASC2 + mimic NC or oe-lncRNA CASC2 + miR-135a-5p mimic by Western blot assay. *P < 0.05

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