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Fig. 2 | Diabetology & Metabolic Syndrome

Fig. 2

From: Long non-coding RNA CASC2 restrains high glucose-induced proliferation, inflammation and fibrosis in human glomerular mesangial cells through mediating miR-135a-5p/TIMP3 axis and JNK signaling

Fig. 2

miR-135a-5p is a target of CASC2. A Bioinformatics database LncBase was utilized to predict CASC2-miRNA interactions, and miR-135a-5p was predicted to be a potential target of CASC2. The complementary sites between miR-135a-5p and CASC2 were shown. B The partial fragment of CASC2, containing the wild-type (wt) or the mutant type (mut) putative binding sites with miR-135a-5p, was amplified and cloned downstream of Firefly luciferase gene in luciferase vector to generate lncRNA CASC2 wt or lncRNA CASC2 mut. C 293T cells were co-transfected with luciferase plasmids and miR-135a-5p mimic or mimic NC, and luciferase activities in four groups were determined to test the interaction between miR-135a-5p and CASC2. D RIP assay was carried out to test the binding relation between miR-135a-5p and CASC2 in HMCs. E The level of miR-135a-5p was examined in HMCs induced by NG or HG for 0 h, 12 h, 24 or 48 h by RT-qPCR. *P < 0.05

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Diabetology & Metabolic Syndrome

ISSN: 1758-5996

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