Fig. 4

Circ-ACTR2/miR-205-5p axis affected the regulation of HG in HRMCs. A The transfection efficiency of anti-miR-205-5p was assessed by qRT-PCR. B The qRT-PCR was applied for the expression detection of miR-205-5p after transfection of si-NC, si-ACTR2, si-ACTR2 + anti-miR-NC or si-ACTR2 + anti-miR-205-5p in HG-treated HRMCs. C Cell viability was examined via CCK-8 assay. D The concentrations of IL-6 and TNF-α were tested via ELSIA. E Cell proliferation was assessed via EdU assay. F, G The levels of collagen I and collagen IV were assayed via western blot. H, I Oxidative stress was evaluated by SOD activity (H) and MDA level (I) using the kits. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001