Fig. 6

PAK1 is a target of miR-889-3p. A The putative miR-889-3p binding sites on the 3′UTR of PAK1. B–D The interaction between miR-889-3p and PAK1 was confirmed using dual luciferase reporter assay, RIP assay and pull-down assay. E qRT-PCR analysis of PAK1 mRNA expression in placental tissues with GDM or not. F The correlation between miR-889-3p and PAK1 expression in placental tissues was analyzed by Pearson analysis. G Western blot analysis of PAK1 protein expression in placental tissues with GDM or not. H Western blot analysis of PAK1 protein expression in HTR8/SVneo cells treated with HG or normal glucose (control) for 48 h. I, J HTR8/SVneo cells were transfected with miR-NC, miR-889-3p, anti-miR-NC, or anti-miR-889-3p and then treated with HG. I qRT-PCR analysis of miR-889-3p level in HTR8/SVneo. J Western blot analysis of PAK1 protein in HTR8/SVneo cells. *P < 0.05, ***P < 0.001, ****P < 0.0001