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Fig. 5 | Diabetology & Metabolic Syndrome

Fig. 5

From: Circ-PNPT1 contributes to gestational diabetes mellitus (GDM) by regulating the function of trophoblast cells through miR-889-3p/PAK1 axis

Fig. 5

MiR-889-3p inhibition attenuates the effects of circ-PNPT1 knockdown on HG-induced trophoblast cells. AF HTR8/SVneo cells were co-transfected with si-circ-PNPT1 and/or anti-miR-889-3p and then subjected to HG stimulation. A qRT-PCR analysis of miR-889-3p expression in cells. B Cell viability analysis with CCK-8 assay. C Cell apoptosis analysis with flow cytometry. D, E Cell migration and invasion abilities analysis using transwell assay. F Wound healing assay for cell migration. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

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