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Fig. 4 | Diabetology & Metabolic Syndrome

Fig. 4

From: Circ-PNPT1 contributes to gestational diabetes mellitus (GDM) by regulating the function of trophoblast cells through miR-889-3p/PAK1 axis

Fig. 4

MiR-889-3p is a target of circ-PNPT1. A The potential binding site between circ-PNPT1 and miR-889-3p. BD The interaction between miR-889-3p and circ-PNPT1 was confirmed using dual luciferase reporter assay, RIP assay and pull-down assay. E qRT-PCR analysis of miR-889-3p expression in placental tissues with GDM or not. F The correlation between miR-889-3p and circ-PNPT1 expression in placental tissues was analyzed by Pearson analysis. G qRT-PCR analysis of miR-889-3p expression in HTR8/SVneo cells treated with HG or normal glucose (control) for 48 h. H qRT-PCR analysis of circ-PNPT1 level in HTR8/SVneo cells transfected with pCD5-ciR or circ-PNPT1 under HG treatment. I qRT-PCR analysis of miR-889-3p expression in HTR8/SVneo cells transfected with pCD5-ciR, circ-PNPT1, si-NC, or si-circ-PNPT1 in the presence of HG. *P < 0.05, ***P < 0.001, ****P < 0.0001

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