Fig. 3From: Circ-PNPT1 contributes to gestational diabetes mellitus (GDM) by regulating the function of trophoblast cells through miR-889-3p/PAK1 axisCirc-PNPT1 knockdown protects trophoblast cell from HG-induced cell growth and transfer processes inhibition. A–F HTR8/SVneo cells were transfected with si-circ-PNPT1 or si-NC and then subjected to HG stimulation. A qRT-PCR analysis of circ-PNPT1 expression in cells. B Cell viability analysis using CCK-8 assay. C Cell apoptosis analysis with flow cytometry. D, E Cell migration and invasion abilities analysis using transwell assay. F Wound healing assay for cell migration. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001Back to article page